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Amy Proal's "Bacteriality.com"
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Prof Trevor Marshall
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 Posted: Sat Apr 26th, 2008 06:23

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The techniique was devised by Lida Mattman. The videos I use (most frequently) were taken by Dr Andrew Wright of the UK using the Bradford microscope at his local university. One of our members, James Sanders, put together a microscope from pieces he bought on Ebay, and produced video you will find discussed on this site. He wrote up a set of slide-prep instructions which were handed out at our LAX conference. They are at URL
http://autoimmunityresearch.org/James_slide_prep.pdf

The Foundation has a microscope as well, and I am working on ways of making it easier for us to sample the pathogens DNA for genomic analysis.
 

jsandman001
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 Posted: Sat Apr 26th, 2008 09:53

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Hello Cocoa,

Im the James that Dr. Marshall mentions above. By the way great job Dr. Marshall, Meg, Amy and Paul.....can we get you guys to put a conference together here in the US :) Just a thought I know I would go!

Anyway back to you Cocoa if you would like me to answer any questions for you I will but dont get to excited yet. I have probably more than 100gigabyte of videos that I made with 3 different microscopes. Stained brightfield, Darkfield,Phase contrast and various other lighting techniques. I have showed these videos to more than a 1000 people most of them were doctors and researchers from many institutions and they were all very excited at first but I have yet to see anyone do anything with what I have found under a microscope.

I learned from Dr. Lida Mattman and Dr. Wright at the Chicago conference Dr. Marshall had. The process is very simple. Dr. Marshall linked you to the slide prep sheet I made up. The thing is you need very deep pockets to go beyond what I , Dr. Wright , Dr. Mattman have done. The next step which I started playing with in my opinion would be antigen specific immunofluorescence to identify the players considering Dr. Mattman has found more than 20 different bacteria that we are playing with. (my reference is a lyme conference speech she did some time back) .......I have gone as far as using fluorescence to show what is in the blood is bacteria which I thought was pretty exciting. The biofilm actually flouresces under the right spectrum of led lighting using prisms on both sides of a slide and strong narrow spectrum led lighting. I would caution anyone with a Th-1 disease.....Leave direct viewing of high intensity microscope lighting to others without a TH-1 disease. Unless you are using a video camera.

The next step would be isolating what we have found and ultimately getting the DNA........which would require big wallets but would lead to the proof that no one could deny about Th-1 illnesses. Especially once the powers that be saw how more alike our illnesses are than not. The only think I think that seperates all of us is what horizontal DNA transfer has taken place and what mutants we have in our lovely bacterial soup but I will defer to Dr. Marshall and Amy's articles on that subject.

For us for now the cure is easier and cheaper than finding the source :) Thanks to Dr. Marshall we have the cure.

Any questions just PM me.

Thanks,

James Sanders



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Aunt Diana
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 Posted: Sat Apr 26th, 2008 09:54

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What an exciting article! I have goosebumps after reading it....thanks so much to all of you for your hard work and dedication.

It feels like something is starting to happen! It sounds like Trevor Marshall was totally ready for any event or opportunity that may have occurred. Fantastic!!



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mndymplol
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 Posted: Sat Apr 26th, 2008 10:03

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Amy,

I was surprised that the Drug Companies were so interested. I guess I assumed that their emphasis would be on maintaining the status quo rather than getting ahead of the curve. I have a friend in the upper eschelons of Phizer. He is a VP now but was one of the researchers who developed Zith. Should I be approaching him in some way about the MP?

Mindy



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Prof Trevor Marshall
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 Posted: Sat Apr 26th, 2008 10:17

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James, IMO we have to head towards single cell sampling and DNA shotgun sequencing. The antibody approach is not very useful because we don't know many of the species involved, and in any case the antibodies tend to be very non-specific, thus labelling many species besides their main target.
 

Ames
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 Posted: Sat Apr 26th, 2008 10:18

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Hi Mindy,

I don't know. It was one man from AstraZeneca that wanted to hear my whole talk and kept asking questions. I can't say why he was so interested, or if other people associated with drug companies would react with the same interest regarding the MP.

Maybe he was tired or hearing the same discussions on genetics and was interested in a fresh perspective. Then again, I do think that he wanted to know what we were doing because if his company decides to invest in lets say, more immunosuppresive drugs, he may tell his associates, "no, there may be a curative treatment option out there, we shouldn't pursue this project." So it's important that he and other people in charge of making drugs know about the MP. That's because if they are planning to put new palliatives out there for chronic disease (at the cost of billions of dollars) and patients don't end up using them because of the MP, they could get seriously screwed.

So I would contact the man you know at Pfizer. A "head's up" may help him out.

Best,

Amy

Last edited on Sat Apr 26th, 2008 10:25 by Ames



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jsandman001
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 Posted: Sat Apr 26th, 2008 10:31

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Dr. marshall,

You are absolutely right although that is going to be a pretty monumental acheivement. I was only stating immunofluorescence as a cheaper way to lend more to what we know......they do have very specific fluorescence but the only way it would be useful at all is to show that yeah the group tests positive for this or that but like I said and we know that we are dealing with many more than 20 for sure types of bacteria then all the mutations.

So when are you going to get the scientists to DNA sequence this stuff :) Im just playing I know what a task it will be.

Anyway my two cents,

James



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migsies
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 Posted: Sat Apr 26th, 2008 14:32

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Thank you Dr.Marshall, Meg, Amy and Paul for bringing such sorely needed attention to the role of pathogens (and subsequent disruption of innate immunity and apoptosis) in mental illness at the 2008 Karolinska conference. Hopefully those with the huge resources required to get on with the much needed DNA work will now be inspired to pick up the ball and maybe collaborate with Dr. Marshall in elucidating the key players (pathogens) involved in chronic illness.

Amy, thanks for the article in bacteriality. It provides the kind of inspiration and hope we all need and lets us know that some 'big shots' in the scientific community could soon be moving in the right direction by exploring the Marshall theory. It also provided some intersting insights into Meg's alternate persona and her love of Irish pubs...something many of us can relate to, at least from before we became ill.

Incidentally, speaking of pathogens and innate immunity, there was an interesting news release recently about Salmonella and how it subverts innate immunity. I can't vouch for the mechanism cited, but at least the news release serves to draw more attention to the critical role of innate immunity and pathogens in disease, which after all is one of the central pillars of the MP theory.:)



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Cocoa
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 Posted: Mon Apr 28th, 2008 04:40

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Dr. Marshall and James,

Thanks for the information about viewing the bacteria. It seems almost inconceivable that such an accessbile technique - ie viewing bacteria in live blood via a microscope - has not resulted in further investigation by other researchers. I do remember reading somewhere - perhaps on Amy's site - that a mainstream researcher(s) believed that such bacteria were only present in live blood because the blood sample had become infected after collection!!!

Regards, Cocoa:)

P.S. "Whenever you find yourself on the side of the majority, it is time to pause and reflect". - Mark Twain



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Ames
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 Posted: Mon May 5th, 2008 21:35

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Thanks Migsies, for you kind words about Bacteriality.  James - it's interesting to learn more about your techniques.

I just put up a short piece on Bacteriality about Dr. Azzuzi's talk at DMM on prions. I hope it simplifies the basics of his speech a little for those who might have had trouble with his accent!  Here's the link:

http://bacteriality.com/2008/05/05/prions/

Amy

PS I've also been working to take the speech I gave about the MP in Sweden and make it into a video.  Actually Paul's working hard on the technical part right now.  It should be up soon.....

Last edited on Mon May 5th, 2008 21:40 by Ames



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Ames
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 Posted: Thu May 8th, 2008 07:30

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Hi!

I just put up my video about the MP.

Here's the description:

In the following video, I explain the science that forms the backbone of the Marshall Protocol in simple terms. I discuss the bacteria implicated in causing chronic inflammatory disease, and explain how these pathogens affect the immune system. I also describe the basics of the treatment itself, providing information that any patient who plans to start the Marshall Protocol, or any doctor planning to put a patient on the MP, should understand. My narration is accompanied by slides with pictures and images of the pathogens and molecules I discuss. The video is 89 minutes.

http://bacteriality.com/2008/05/07/mpintro/

Best,

Amy



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Ames
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 Posted: Thu May 8th, 2008 12:06

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I have temporarily taken down the video because the audio for a few slides was messed up and needs to be corrected.  It should be up later tonight.....



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jrfoutin
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 Posted: Thu May 8th, 2008 15:31

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Eeek! I just sent the link to about 500 people.... OK. Maybe only 50, but the day is young ;)

Why not put the link back up even with the rough cut until you have a replacement so people can see it? I'd hate to have sent them there only to see it down, and then they give up and never come back. At least the rough cut will allow them access to content in a timely fashion.

Thanks!--Janet



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NorCalJim
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 Posted: Thu May 8th, 2008 15:41

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I caught the entire first draft video and noticed a couple sections seemed to be repeated and a couple slides seemd to be reversed. Other than that though it was absolutely awesome!!! Any plans to burn this to DVD?

NorCalJim



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jrfoutin
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 Posted: Thu May 8th, 2008 15:48

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Maybe you don't think it is ready for the Sundance film festival


. . . ;) (yet) ;) . . .


but I think your presentation was wonderful.


Saw the full first version and even with a few minor issues, the content is good thinking, excellent content in a reasonable amount of time. 

Please put the rough cut up until you replace it with your polished expectations.

Thanks!--Janet



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Martin78
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 Posted: Thu May 8th, 2008 16:13

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Hi Ames

I managed to see it before you took it down and yes, at about 15, 43 and 50something minutes there are some audio cuts that repeat themselves.

The presentation is good. If I were to give an advice on how to broaden the usage of the clip, I would divide it into shorter chapters.

Like: (this is just examples of themes, I dont remeber the storyline well enough to make proper suggestions for actual chapters)

1: What is the background (pathelogical, history, other scientists works microbs)

2; The science

3: Vitamin D

4: The actual protocol/Clinical "how to do"

If all these "chapters" where about 15-20 minutes long, It could maybee  be easier for patients to bring their lap top to their doctor and show them bit by bit whats its all about.

Before I started the MP I brought my laptop to my doctor and showed her Dr. Blaneys "Histology" speach from the DVD, and compared the findings he had experienced from his clinical practise with th1 patients and my own symptoms/findings, witch took about 20 minutes, and I think my doc appriciated it.

Because I could show her that I also had prolapsed disc, acid reflux, toenail candida, swalloing issues, skin rashes, irritability attacks, positive for mono etc ect.. all the thypical findings that Dr. Blaney mentions, I believe it was easier for my Doc to conclude that the MP was appropriate treatment.

Off course these findings came in addition to my hypercalcimia and 1,25 values off the roof. But many patients might not be so "lucky" to have so clear hypervitaminonis as I did have and might need more tools in their box to be given the treatment. Furthermore the population study used on the study site can be questioned by clinicians, They can show populations studies (with equally high "n",  with a much higher mean value of both 025 and 1,25 than the one used here.  This again would lead to much lower sigma numbers when used as baseline, buts thats another story.

Now, I am lucky, because I can have 1 hour (60min) consultations, but not everyone can, so breaking the "movie" down, little by little, would maybe be a good idea for this use.

For the rest of us (who has more time due to our illness and gladly spends 89 minutes infront a computer) the "full night movie version" works just fine.

Just my thoughts. Keep it up! You are doing a great job.

Kind Regards

Martin

 



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paulalbert
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 Posted: Thu May 8th, 2008 16:27

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So sorry for the denials of service people! I've published that page again even with the crappy version.

We have been scrambling to put out a new version, and it should be ready by 7pm EST, Lord willing.

Meanwhile, I'm uploading the original quicktime movie to a sharing site in case any of you want to see a better quality presentation. I'll be publishing a link to that in this space.

If you have any problems, please email me-- palbert1@gmail.com

Paul



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Prof Trevor Marshall
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 Posted: Thu May 8th, 2008 16:57

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I will shamelessly take advantage of this high-traffic page :) to make sure everybody has seen the Guidance for Emergency Room Personnel that Meg has put online today. Print a copy of the PDF and keep it in the glove box of your car...

http://www.marshallprotocol.com/forum18/12026.html
 

Ames
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 Posted: Thu May 8th, 2008 18:08

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The edited version of the video is now up again!  Please let me know if you see any other mistakes.

http://bacteriality.com/2008/05/07/mpintro/

For those who want to download a high resolution of the video, click on the "Vimeo" link in the videoplayer.  There is a download button on the bottom right of the webpage.  The file is currently at about 150 megabites.  Please note that the latest version of this video will always be embedded on the Bacteriality website, as it's likely the video content may be changed slightly in the future if new discoveries are made or new papers released.

By the way, be sure to read the post above this one where Dr. Marshall has posted the link for the new Guidelines for Emergency Room Personnel.

Best,

Amy

PS  Martin, sorry, I was writing so quickly I forget to address your suggestions.  While I think it would be helpful to some to break the video into sections I did try to make it flow as one piece.  I tried to interconnect some of what I say at the start and gradually weave a picture of how the MP parts fit together over the video. 

I could still break it up into four videos, although the vitamin D part wouldn't be complete as I spread it throught the video at times.

Since Paul and I will be in Chicago for the next two weeks on vacation, hopefully for now people could remember the time in the video where a certain section they want their physician or a friend to see starts and fast-forward to that point. 

In the meantime, if other people would like the video broken down into sections please let me know, here or by email so I can see what the demand is like or if people prefer it whole.

Thanks!

Amy

Last edited on Thu May 8th, 2008 19:40 by Ames



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Prof Trevor Marshall
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 Posted: Thu May 8th, 2008 20:28

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There is a program called AVIdemux (open source) which will take the MOV file you can download from Vimeo's website and cut it up with start and stop marks where you want them, copying that segment out into an MP4 container for either Windoze, Mac or Linux (or even sending back to Vimeo). Surely there is a 'volunteer' here who could do that, and reduce the load on Amy and Paul?

http://avidemux.sourceforge.net/
 


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